[Cytotoxicity of two dental materials on fibroblasts derived from human embryonic stem cells]

Beijing Da Xue Xue Bao Yi Xue Ban. 2012 Feb 18;44(1):1-5.
[Article in Chinese]


Objective: To investigate the cytotoxicity of calcium hydroxide (CH) and composite resin on fibroblasts derived from human embryo body fibroblasts-H9 (EBf-H9), human dental pulp cells (hDPCs) and immortalized fibroblasts L929; and to evaluate the use of EBf-H9 as a cellular model for cytotoxicity screening of dental materials.

Methods: The EBf-H9 cells were derived from human embryonic stem cells (H9) via outgrowth of embryonic body (EB); hDPCs were isolated from healthy dental pulp, and identified by immunochemical staining. Cell Counting Kit-8 (CCK-8) assay was applied to analyze the cytotoxicity of CH and composite resin with serial concentrations on the 3 kinds of cells.

Results: Following 24 h and 48 h (or 72 h) post-treatment of CH and composite resin, the viability of L929 cells was significantly lower than that of EBf-H9 and hDPCs (P<0.05), and there was no significant difference between the last two groups (P>0.05).

Conclusion: Immortalized fibroblasts L929 cells exhibited different response to CH and composite resin compared with EBf-H9 and hDPCs, and the last two cell types were similar to each other. This study indicated that fibroblasts derived from human embryonic stem cells were a potential cellular model instead of traditional immortalized murine cell line for cytotoxicity screening assay.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium Hydroxide / toxicity*
  • Cells, Cultured
  • Composite Resins / toxicity*
  • Dental Materials / toxicity*
  • Dental Pulp / cytology
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / drug effects*
  • Fibroblasts / cytology
  • Fibroblasts / drug effects*
  • Humans
  • Materials Testing
  • Mice
  • Toxicity Tests


  • Composite Resins
  • Dental Materials
  • Calcium Hydroxide