Systemic aminoglycosides are trafficked via endolymph into cochlear hair cells

Sci Rep. 2011;1:159. doi: 10.1038/srep00159. Epub 2011 Nov 16.

Abstract

Aminoglycoside antibiotics rapidly enter and kill cochlear hair cells via apical mechanoelectrical transduction (MET) channels in vitro. In vivo, it remains unknown whether systemically-administered aminoglycosides cross the blood-labyrinth barrier into endolymph and enter hair cells. Here we show, for the first time, that systemic aminoglycosides are trafficked across the blood-endolymph barrier and preferentially enter hair cells across their apical membranes. This trafficking route is predominant compared to uptake via hair cell basolateral membranes during perilymph infusion.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoglycosides / administration & dosage
  • Aminoglycosides / pharmacokinetics*
  • Aminoglycosides / toxicity
  • Animals
  • Biological Transport, Active
  • Endolymph / metabolism*
  • Female
  • Fluorescent Dyes
  • Gentamicins / administration & dosage
  • Gentamicins / pharmacokinetics
  • Gentamicins / toxicity
  • Guinea Pigs
  • Hair Cells, Auditory / drug effects
  • Hair Cells, Auditory / metabolism*
  • Male
  • Models, Biological
  • Perilymph / metabolism
  • Xanthenes

Substances

  • Aminoglycosides
  • Fluorescent Dyes
  • Gentamicins
  • Xanthenes
  • Texas red