Cdc14b regulates mammalian RNA polymerase II and represses cell cycle transcription

Sci Rep. 2011;1:189. doi: 10.1038/srep00189. Epub 2011 Dec 12.

Abstract

Cdc14 is an essential phosphatase in yeast but its role in the mammalian cell cycle remains obscure. We report here that Cdc14b-knockout cells display unscheduled induction of multiple cell cycle regulators resulting in early entry into DNA replication and mitosis from quiescence. Cdc14b dephosphorylates Ser5 at the C-terminal domain (CTD) of RNA polymerase II, a major substrate of cyclin-dependent kinases. Lack of Cdc14b results in increased CTD-Ser5 phosphorylation, epigenetic modifications that mark active chromatin, and transcriptional induction of cell cycle regulators. These data suggest a function for mammalian Cdc14 phosphatases in the control of transcription during the cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Binding Sites
  • Cell Cycle*
  • Cells, Cultured
  • Dual-Specificity Phosphatases / metabolism*
  • Epigenesis, Genetic
  • Exons
  • Fibroblasts / cytology
  • Gene Expression Regulation, Enzymologic
  • Mice
  • Mitosis
  • Phosphates / chemistry
  • Phosphorylation
  • RNA Polymerase II / metabolism*
  • Transcription, Genetic*
  • Transcriptional Activation

Substances

  • Phosphates
  • RNA Polymerase II
  • CDC14B protein, mouse
  • Dual-Specificity Phosphatases