Development of a transcription-reverse transcription concerted reaction method for specific detection of human enterovirus 71 from clinical specimens

J Clin Microbiol. 2012 May;50(5):1764-8. doi: 10.1128/JCM.00394-12. Epub 2012 Feb 22.

Abstract

A transcription-reverse transcription (RT) concerted reaction (TRCR) method was developed for rapid and specific detection of EV71 from clinical specimens. This method was validated with EV71 strains from all of the known genotypes (genotypes A, B1 to B5, and C1 to C5), with detection limits of 10 to 10(3) copies, and was useful for identification of EV71 from throat swabs of patients with hand, foot, and mouth disease (HFMD).

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Enterovirus A, Human / genetics
  • Enterovirus A, Human / isolation & purification*
  • Hand, Foot and Mouth Disease / diagnosis*
  • Hand, Foot and Mouth Disease / virology
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Pharynx / virology
  • RNA, Viral / genetics*
  • Reverse Transcription*
  • Sensitivity and Specificity
  • Transcription, Genetic*
  • Virology / methods*

Substances

  • RNA, Viral