Generation of lymphokine activated killer cells in a new high density dialyzing culture apparatus

Y Kohgo; S Sakamaki; Y Kanisawa; S Nojiri; Y Ueno; Y Itoh; M Takahashi; Y Sasagawa; S Hosoi; S Sato; Y Niitsu

doi:10.1007/BF00365414

Generation of lymphokine activated killer cells in a new high density dialyzing culture apparatus

Cytotechnology. 1989 Feb;2(1):49-57. doi: 10.1007/BF00365414.

Authors

Y Kohgo¹, S Sakamaki, Y Kanisawa, S Nojiri, Y Ueno, Y Itoh, M Takahashi, Y Sasagawa, S Hosoi, S Sato, Y Niitsu

Affiliation

¹ Fourth Department of Internal Medicine, Sapporo Medical College, South-1, West-16, 060, Sapporo, Japan.

PMID: 22358580
DOI: 10.1007/BF00365414

Abstract

In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conventional culture conditions. By using this new apparatus with continuous regulation of infused interleukin 2, nutritional medium, and pO(2) and pCO(2), yields of 2×10(7) cells/ml were achieved and maintained for more than 21 days. These cells also showed a significant increase of LAK activity on a per cell basis.