Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization

H Tanaka; N Fukuda; Y Shoyama

doi:10.1023/A:1008068718392

Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization

Cytotechnology. 1999 Mar;29(2):115-20. doi: 10.1023/A:1008068718392.

Authors

H Tanaka¹, N Fukuda, Y Shoyama

Affiliation

¹ Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812, Japan.

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Rc and ginsenoside Rd. The full measuring range of the assay extends from 20 ngml-1 to 400 ngml-1 of ginsenoside Rb1.