ISGF3, the transcriptional activator induced by interferon alpha, consists of multiple interacting polypeptide chains

Proc Natl Acad Sci U S A. 1990 Nov;87(21):8555-9. doi: 10.1073/pnas.87.21.8555.

Abstract

Interferon-stimulated gene factor 3 (ISGF3) is the ligand-dependent transcriptional activator that, in response to interferon treatment, is assembled in the cell cytoplasm, is translocated to the nucleus, and binds the consensus DNA site, the interferon-stimulated response element. We have purified ISGF3 and identified its constituent proteins: a DNA-binding protein of 48 kDa and three larger polypeptides (84, 91, and 113 kDa), which themselves do not have DNA-binding activity. The multisubunit structure of ISGF3 most likely reflects its participation in receiving a ligand-dependent signal, translocating to the nucleus, and binding to DNA to activate transcription.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Chromatography, Affinity
  • Chromatography, Ion Exchange
  • DNA
  • DNA-Binding Proteins / isolation & purification*
  • DNA-Binding Proteins / metabolism
  • HeLa Cells / drug effects
  • HeLa Cells / metabolism
  • Humans
  • Interferon Type I / pharmacology*
  • Interferon-Stimulated Gene Factor 3
  • Interferon-Stimulated Gene Factor 3, gamma Subunit
  • Macromolecular Substances
  • Molecular Sequence Data
  • Molecular Weight
  • Oligodeoxyribonucleotides
  • Transcription Factors / isolation & purification*
  • Transcription Factors / metabolism
  • Transcription, Genetic*

Substances

  • DNA-Binding Proteins
  • IRF9 protein, human
  • Interferon Type I
  • Interferon-Stimulated Gene Factor 3
  • Interferon-Stimulated Gene Factor 3, gamma Subunit
  • Macromolecular Substances
  • Oligodeoxyribonucleotides
  • Transcription Factors
  • DNA