Abstract
A case study of the bioremediation of groundwater contaminated with trichloroethene (TCE) was conducted using the biostimulation agent, BD-1. TCE levels were monitored by gas chromatography-mass spectroscopy. Total organic carbon (TOC) and volatile fatty acids (VFAs) were analyzed to investigate the environmental fate of BD-1. The effects of BD-1 on microbial activity were investigated using 16S rRNA gene-based quantitative polymerase chain reaction (qPCR) analysis. The biodegradation of BD-1 was accompanied by a reduction in TCE, and the initially high TOC levels decreased rapidly as BD-1 was transformed into VFAs. qPCR analysis showed that the genus Dehalobacter became progressively dominant through the experiment. These results suggested that BD-1 might dechlorinate TCE by activating dechlorinating bacteria.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteria / drug effects
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Bacteria / genetics
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Biocompatible Materials / pharmacology
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Biodegradation, Environmental / drug effects
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Carbon Dioxide / analysis
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Denaturing Gradient Gel Electrophoresis
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Fatty Acids, Monounsaturated / chemistry
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Fatty Acids, Monounsaturated / pharmacology*
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Fatty Acids, Volatile / analysis
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Groundwater / chemistry*
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Groundwater / microbiology
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Halogenation* / drug effects
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Hydrogen / analysis
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Japan
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Molecular Sequence Data
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Oxidation-Reduction / drug effects
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RNA, Ribosomal, 16S / genetics
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Rapeseed Oil
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Trichloroethylene / isolation & purification*
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Water Pollutants, Chemical / isolation & purification*
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Water Pollution / analysis*
Substances
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BD-1 biostimulation agent
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Biocompatible Materials
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Fatty Acids, Monounsaturated
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Fatty Acids, Volatile
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RNA, Ribosomal, 16S
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Rapeseed Oil
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Water Pollutants, Chemical
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Carbon Dioxide
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Trichloroethylene
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Hydrogen
Associated data
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GENBANK/AB517889
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GENBANK/AB517890
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GENBANK/AB517891
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GENBANK/AB517892
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GENBANK/AB517893
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GENBANK/AB517894
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GENBANK/AB517895
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GENBANK/AB517896
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GENBANK/AB517897
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GENBANK/AB517898
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GENBANK/AB517899
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GENBANK/AB517900
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GENBANK/AB517901
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GENBANK/AB517902
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GENBANK/AB517903
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GENBANK/AB517904
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GENBANK/AB517905
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GENBANK/AB517906
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GENBANK/AB517907
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GENBANK/AB517908
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GENBANK/AB517909
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GENBANK/AB517910
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GENBANK/AB517911
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GENBANK/AB517912
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GENBANK/AB517913
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GENBANK/AB517914
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GENBANK/AB517915
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GENBANK/AB517916
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GENBANK/AB517917
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GENBANK/AB517918
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GENBANK/AB517919