Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2012 Feb 24;45(4):567-80.
doi: 10.1016/j.molcel.2011.12.034.

Dynamic Protein-Protein Interaction Wiring of the Human Spliceosome

Affiliations

Dynamic Protein-Protein Interaction Wiring of the Human Spliceosome

Anna Hegele et al. Mol Cell. .

Abstract

More than 200 proteins copurify with spliceosomes, the compositionally dynamic RNPs catalyzing pre-mRNA splicing. To better understand protein - protein interactions governing splicing, we systematically investigated interactions between human spliceosomal proteins. A comprehensive Y2H interaction matrix screen generated a protein interaction map comprising 632 interactions between 196 proteins. Among these, 242 interactions were found between spliceosomal core proteins and largely validated by coimmunoprecipitation. To reveal dynamic changes in protein interactions, we integrated spliceosomal complex purification information with our interaction data and performed link clustering. These data, together with interaction competition experiments, suggest that during step 1 of splicing, hPRP8 interactions with SF3b proteins are replaced by hSLU7, positioning this second step factor close to the active site, and that the DEAH-box helicases hPRP2 and hPRP16 cooperate through ordered interactions with GPKOW. Our data provide extensive information about the spliceosomal protein interaction network and its dynamics.

Similar articles

See all similar articles

Cited by 137 articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

Feedback