Abstract
Direct extraction of Cryptosporidium DNA from 46 stools by bead-beating, guanidine thiocyanate and silica purification provided slightly lower PCR positivity (93.5% vs. 100%) and higher threshold cycle values (mean 34.93 vs. 28.03; P=0.00) than spin-column extraction from boiled, semi-purified oocyst suspensions. However, direct extraction is cheaper, and amenable to automation.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Clinical Laboratory Techniques / methods*
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Cryptosporidiosis / diagnosis*
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Cryptosporidiosis / parasitology
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Cryptosporidium / genetics
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Cryptosporidium / isolation & purification*
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DNA, Protozoan / isolation & purification*
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Feces / parasitology*
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Humans
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Parasitology / methods*
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Real-Time Polymerase Chain Reaction / methods*
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Sensitivity and Specificity