Removing endotoxin from plasmid samples by Triton X-114 isothermal extraction

Anal Biochem. 2012 May 15;424(2):124-6. doi: 10.1016/j.ab.2012.02.015. Epub 2012 Feb 25.


Triton X-114 temperature transition extraction has been considered to be a simple and cost-effective strategy to eliminate endotoxin from plasmid preparations. However, a repeated cooling-heating process may promote the degradation of plasmid DNA. Based on the finding that the cloud point of Triton X-114 solution increases substantially in the presence of small amounts of sodium dodecyl sulfate (SDS) and that electrolytes decrease the cloud point of Triton X-114-SDS solution drastically, we designed a Triton X-114 isothermal extraction method for removing endotoxin from plasmid samples and found that it has the same endotoxin removal efficiency when compared with the temperature transition extraction method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / isolation & purification*
  • Detergents / chemistry
  • Endotoxins / isolation & purification*
  • Octoxynol
  • Phase Transition
  • Plasmids / isolation & purification*
  • Polyethylene Glycols / chemistry*
  • Sodium Chloride / chemistry
  • Sodium Dodecyl Sulfate / chemistry
  • Temperature


  • Detergents
  • Endotoxins
  • Sodium Dodecyl Sulfate
  • Polyethylene Glycols
  • Sodium Chloride
  • Octoxynol
  • DNA
  • Nonidet P-40