Amplifications of NCOA3 gene in colorectal cancers in a Chinese population

World J Gastroenterol. 2012 Feb 28;18(8):855-60. doi: 10.3748/wjg.v18.i8.855.

Abstract

Aim: To investigate the copy number variation of NACO3 gene in colorectal cancer (CRC) and its correlation with tumor progression.

Methods: A total of 142 samples of case-matched CRC tissues and adjacent normal tissues were obtained from patients undergoing bowel resection. Quantitative real-time polymerase chain reaction method was used to investigate the copy number variations of NCOA3 as well as gene expression in the collected tissues.

Results: Copy number gains of NCOA3 were detected in 39 CRC samples (27.5%) and were correlated with tumor progression (χ2 = 6.42, P = 0.0112). Moreover, there was a positive correlation between copy number gain and mRNA over-expression of NCOA3 in CRCs (P = 0.0023). Expression level of NCOA3 mRNA was also enhanced in the CRC samples with unaltered copy numbers (3.85 ± 1.23 vs. 2.71 ± 0.64, P < 0.01).

Conclusion: Sporadic colorectal cancers exhibit different mechanisms of NCOA3 regulation.

Keywords: Colorectal cancer; Gene copy number; Gene expression; NCOA3; Tumor progression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Asians / genetics*
  • Colorectal Neoplasms / genetics*
  • Colorectal Neoplasms / pathology*
  • DNA Copy Number Variations*
  • Disease Progression
  • Gene Amplification*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Nuclear Receptor Coactivator 3 / genetics*

Substances

  • NCOA3 protein, human
  • Nuclear Receptor Coactivator 3