Clinical significance of circulating miR-126 quantification in malignant mesothelioma patients

Clin Biochem. 2012 May;45(7-8):575-81. doi: 10.1016/j.clinbiochem.2012.02.009. Epub 2012 Feb 21.


Objectives: Aim of this study was to evaluate the accuracy and precision of the detection of individual miRNA as clinical biomarkers in the serum.

Design and methods: miRNA-126 was quantified in serum using endogenous and exogenous controls for normalization and the accuracy and precision of the method evaluated. The diagnostic value of serum miRNA-126 was evaluated in malignant mesothelioma (MM) and non-small-cell lung cancer (NSCLC) patients using both relative and absolute qRT-PCR methods.

Results: The use of endogenous invariant and exogenous synthetic controls as well sample dilution markedly improves the accuracy and precision of the assay. The inter- and intra-assay analyses revealed that relative qRT-PCR is a more reliable method. Circulating miR-126 detected in the serum by relative qRT-PCRs was found low-expressed in both malignancies, significantly differentiated MM patients from healthy controls and NSCLC from MM, but do not discriminate NSCLC patients from control subjects. Kaplan-Meier analysis revealed that low level of circulating miR-126 in MM patients was strongly associated with worse prognosis.

Conclusions: We propose that this approach can be adopted for accurate analysis of other suitable circulating miRNA markers of different types of cancer.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Biomarkers, Tumor / blood
  • Biomarkers, Tumor / genetics
  • Carcinoma, Non-Small-Cell Lung / blood
  • Carcinoma, Non-Small-Cell Lung / diagnosis
  • Carcinoma, Non-Small-Cell Lung / genetics
  • Case-Control Studies
  • Female
  • Humans
  • Kaplan-Meier Estimate
  • Male
  • Mesothelioma / blood
  • Mesothelioma / diagnosis*
  • Mesothelioma / genetics
  • MicroRNAs / analysis
  • MicroRNAs / blood*
  • Middle Aged
  • Prognosis
  • Reference Standards
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Sensitivity and Specificity


  • Biomarkers, Tumor
  • MIRN126 microRNA, human
  • MicroRNAs