The Conserved Oligomeric Golgi Complex Is Required for Fucosylation of N-glycans in Caenorhabditis Elegans

Glycobiology. 2012 Jun;22(6):863-75. doi: 10.1093/glycob/cws053. Epub 2012 Feb 28.

Abstract

The conserved oligomeric Golgi complex (COG) is a hetero-octomeric peripheral membrane protein required for retrograde vesicular transport and glycoconjugate biosynthesis within the Golgi. Mutations in subunits 1, 4, 5, 6, 7 and 8 are the basis for a rare inheritable human disease termed congenital disorders of glycosylation type-II. Defects to COG complex function result in aberrant glycosylation, protein trafficking and Golgi structure. The cellular function of the COG complex and its role in protein glycosylation are not completely understood. In this study, we report the first detailed structural analysis of N-glycans from a COG complex-deficient organism. We employed sequential ion trap mass spectrometry of permethylated N-glycans to demonstrate that the COG complex is essential for the formation of fucose-rich N-glycans, specifically antennae fucosylated structures in Caenorhabditis elegans. Our results support the supposition that disruption to the COG complex interferes with normal protein glycosylation in the medial and/or trans-Golgi.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Caenorhabditis elegans / chemistry
  • Caenorhabditis elegans / metabolism*
  • Glycosylation
  • Golgi Apparatus / chemistry*
  • Golgi Apparatus / metabolism*
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / metabolism*
  • Polysaccharides / chemistry
  • Polysaccharides / metabolism*

Substances

  • Membrane Glycoproteins
  • Polysaccharides