Chemoprevention with green propolis green propolis extracted in L-lysine versus carcinogenesis promotion with L-lysine in N-Butyl-N-[4-hydroxybutyl] nitrosamine (BBN) induced rat bladder cancer

Acta Cir Bras. 2012 Feb;27(2):185-92. doi: 10.1590/s0102-86502012000200015.


Purpose: To determine the effects of green propolis extracted in L-lysine (WSDP) and of L- lysine for 40 weeks on induced rat bladder carcinogenesis.

Methods: The animals (groups I, II, III, IV, V and VI) received BBN during 14 weeks. Group I was treated with propolis 30 days prior received BBN, and then these animals were treated daily with propolis; Groups II and III was treated with subcutaneous and oral propolis (respectively) concurrently with BBN. The animals of Group IV were treated L-lysine; Group V received water subcutaneous; and Group VI received only to BBN. Among the animals not submitted to carcinogenesis induction, Group VII received propolis, Group VIII received L-lysine and Group IX received water.

Results: The carcinoma incidence in Group I was lower than that of control (Group VI). The carcinoma multiplicity in Group IV was greater than in Group VI. All animals treated with L-lysine developed carcinomas, and they were also more invasive in Group IV than in controls. On the other hand, Group VIII showed no bladder lesions.

Conclusion: The WSDP is chemopreventive against rat bladder carcinogenesis, if administered 30 days prior to BBN , and that L-lysine causes promotion of bladder carcinogenesis.

MeSH terms

  • Animals
  • Anticarcinogenic Agents / pharmacology
  • Anticarcinogenic Agents / therapeutic use
  • Butylhydroxybutylnitrosamine / therapeutic use*
  • Carcinogens
  • Female
  • Lysine / pharmacology*
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Propolis / pharmacology
  • Propolis / therapeutic use*
  • Rats
  • Rats, Wistar
  • Time Factors
  • Treatment Outcome
  • Urinary Bladder Neoplasms / chemically induced
  • Urinary Bladder Neoplasms / drug therapy
  • Urinary Bladder Neoplasms / pathology
  • Urinary Bladder Neoplasms / prevention & control*


  • Anticarcinogenic Agents
  • Carcinogens
  • Plant Extracts
  • Butylhydroxybutylnitrosamine
  • Propolis
  • Lysine