[Determination by High Performance Chromatography, Steroid Saponins in a Biologically Active Food Supplements Containing the Extract of Tribulus Terrestris]

Vopr Pitan. 2011;80(6):67-71.
[Article in Russian]


Steroidal saponins are bioactive substances of Tribulus terrestris and can be used to assess the quality of raw materials and processed products from them. For this purpose has been developed the method of qualitative and quantitative determination of steroidal saponins by high performance liquid chromatography with spectrophotometric and mass-selective detection and optimal conditions of sample preparation (70% methanol extraction with sonication and heating); also has been studied steroidal saponins composition of Tribulus terrestris (protodioscin, tribulosaponin B, metilprotodiostsin, terrestrozin H, prototribestin, gracillin and others were found).

Publication types

  • English Abstract

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Dietary Supplements / analysis*
  • Mass Spectrometry
  • Plant Components, Aerial / chemistry
  • Plant Extracts / analysis
  • Plant Extracts / chemistry
  • Plants, Medicinal / chemistry
  • Saponins / analysis*
  • Saponins / chemistry
  • Steroids / analysis*
  • Steroids / chemistry
  • Tribulus / chemistry*


  • Plant Extracts
  • Saponins
  • Steroids