Anatase-based implants nanocoating on stem cells derived from adipose tissue

Implant Dent. 2012 Apr;21(2):118-23. doi: 10.1097/ID.0b013e31824bc948.

Abstract

Purpose: The aim of this study was to investigate the effect of a new anatase coating with antibacterial properties (Bactercline anatase coating [BAC]) on dental implants in the commitment of stem cells derived from adipose tissue to osteoblasts.

Materials and methods: Using real-time reverse transcription polymerase chain reaction, the quantitative expression of specific genes, such as transcriptional factors (runx2 and sp7), bone-related genes (spp1, col1a1, col3a1, alpl, and fosl1), and mesenchymal stem cells marker (eng), was examined.

Results: BAC caused induction of bone-related genes such as sp7, fosl1, alpl, and spp1. In contrast, the expression of runx2, col3a1, and col1a1 was decreased in stem cells treated with BAC with respect to untreated cells.

Conclusion: The obtained results are relevant to better understand the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / cytology*
  • Adult
  • Alkaline Phosphatase / analysis
  • Antigens, CD / analysis
  • Biomarkers / analysis
  • Bone Marrow Cells / cytology
  • Cell Culture Techniques
  • Cell Differentiation / physiology
  • Cell Proliferation
  • Coated Materials, Biocompatible / chemistry*
  • Collagen Type I / analysis
  • Collagen Type I, alpha 1 Chain
  • Collagen Type III / analysis
  • Core Binding Factor Alpha 1 Subunit / analysis
  • Dental Implants*
  • Endoglin
  • Humans
  • Materials Testing
  • Mesenchymal Stem Cells / cytology
  • Nanostructures / chemistry*
  • Osteoblasts / cytology
  • Osteogenesis / physiology
  • Osteopontin / analysis
  • Proto-Oncogene Proteins c-fos / analysis
  • Real-Time Polymerase Chain Reaction
  • Receptors, Cell Surface / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sp7 Transcription Factor
  • Stem Cells / cytology*
  • Stromal Cells / cytology
  • Titanium / chemistry*
  • Transcription Factors / analysis

Substances

  • Antigens, CD
  • Biomarkers
  • COL3A1 protein, human
  • Coated Materials, Biocompatible
  • Collagen Type I
  • Collagen Type I, alpha 1 Chain
  • Collagen Type III
  • Core Binding Factor Alpha 1 Subunit
  • Dental Implants
  • ENG protein, human
  • Endoglin
  • Proto-Oncogene Proteins c-fos
  • RUNX2 protein, human
  • Receptors, Cell Surface
  • SPP1 protein, human
  • Sp7 Transcription Factor
  • SP7 protein, human
  • Transcription Factors
  • fos-related antigen 1
  • Osteopontin
  • titanium dioxide
  • Titanium
  • ALPL protein, human
  • Alkaline Phosphatase