Benzophenone synthase from Garcinia mangostana L. pericarps

Phytochemistry. 2012 May;77:60-9. doi: 10.1016/j.phytochem.2012.02.002. Epub 2012 Mar 3.


The cDNA of a benzophenone synthase (BPS), a type III polyketide synthase (PKS), was cloned and the recombinant protein expressed from the fruit pericarps of Garcinia mangostana L., which contains mainly prenylated xanthones. The obtained GmBPS showed an amino acid sequence identity of 77-78% with other plant BPSs belonging to the same family (Clusiaceae). The recombinant enzyme produced 2,4,6-trihydroxybenzophenone as the predominant product with benzoyl CoA as substrate. It also accepted other substrates, such as other plant PKSs, and used 1-3 molecules of malonyl CoA to form various phloroglucinol-type and polyketide lactone-type compounds. Thus, providing GmBPS with various substrates in vivo might redirect the xanthone biosynthetic pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carbon-Carbon Ligases / chemistry*
  • Carbon-Carbon Ligases / genetics
  • Carbon-Carbon Ligases / isolation & purification
  • Chromatography, High Pressure Liquid
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • Escherichia coli / genetics
  • Garcinia mangostana / enzymology*
  • Garcinia mangostana / genetics
  • Kinetics
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Phylogeny
  • Plant Proteins / chemistry*
  • Plant Proteins / genetics
  • Plant Proteins / isolation & purification
  • Sequence Alignment
  • Sequence Analysis, Protein
  • Substrate Specificity


  • DNA, Complementary
  • Plant Proteins
  • Carbon-Carbon Ligases
  • benzophenone synthase