Screening of integrin-binding peptides from the laminin α4 and α5 chain G domain peptide library

Arch Biochem Biophys. 2012 May;521(1-2):32-42. doi: 10.1016/j.abb.2012.02.017. Epub 2012 Mar 3.

Abstract

Laminins, a multifunctional protein family of extracellular matrix, interact with various types of integrin. Here, integrin-mediated cell adhesive peptides have been systematically screened in the laminin α4 and α5 chain G domain peptide library consisting of 211 peptides by both the peptide-coated plastic plates and peptide-conjugated Sepharose bead assays using human dermal fibroblasts. Thirteen peptides promoted cell spreading and the activity was specifically inhibited by EDTA. Cell attachment to 11 peptides was inhibited by anti-integrin β1 antibody. Additionally, cell attachment to the A5G81 (AGQWHRVSVRWG) and A5G84 (TWSQKALHHRVP) peptides was specifically inhibited by anti-integrin α3 and α6 antibodies. These results suggest that the A5G81 and A5G84 peptides promote integrin α3β1- and α6β1-mediated cell attachment. Further, most of the integrin-mediated cell adhesive peptides are located in the loop regions in the G domains, suggesting that structure is important for the integrin specific recognition. Integrin binding peptides are useful for understanding laminin functions and have a potential to use for biomaterials and drug development.

MeSH terms

  • Actin Cytoskeleton / metabolism
  • Amino Acid Sequence
  • Animals
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Adhesion
  • Cells, Cultured
  • Fibroblasts / metabolism
  • Focal Adhesion Kinase 1 / metabolism
  • Humans
  • Integrin alpha3 / metabolism
  • Integrin alpha6 / metabolism
  • Integrin beta1 / metabolism
  • Integrins / metabolism*
  • Laminin / chemistry
  • Laminin / genetics
  • Laminin / metabolism*
  • Mice
  • Molecular Sequence Data
  • Peptide Fragments / chemistry
  • Peptide Fragments / genetics
  • Peptide Fragments / metabolism
  • Peptide Library
  • Protein Structure, Tertiary
  • Vinculin / metabolism

Substances

  • Carrier Proteins
  • Integrin alpha3
  • Integrin alpha6
  • Integrin beta1
  • Integrins
  • Lama4 protein, mouse
  • Laminin
  • Peptide Fragments
  • Peptide Library
  • VCL protein, human
  • laminin alpha5
  • Vinculin
  • Focal Adhesion Kinase 1
  • PTK2 protein, human