OBJECTIVE To identify whether calcium, vitamin D, and/or dairy intakes are prospectively associated with stress fracture risk among female adolescents. DESIGN Prospective cohort study. SETTING Adolescent girls living throughout the United States. PARTICIPANTS A total of 6712 girls aged 9 to 15 years at baseline in the Growing Up Today Study, an ongoing prospective cohort study. MAIN EXPOSURES Dairy, calcium, and vitamin D intakes assessed by food frequency questionnaire every 12 to 24 months between 1996 and 2001. MAIN OUTCOME MEASURE Incident stress fracture that occurred between 1997 and 2004 as reported by mothers of the participants in 2004. Cox proportional hazards models were used to examine associations. RESULTS During 7 years of follow-up, 3.9% of the girls developed a stress fracture. Dairy and calcium intakes were unrelated to risk of developing a stress fracture. However, vitamin D intake was inversely related to stress fracture risk. The multivariable-adjusted hazard ratio of stress fracture for the highest vs the lowest quintile of vitamin D intake was 0.49 (95% CI, 0.24-1.01; Ptrend = .07). We conducted a stratified analysis to estimate the association between vitamin D intake and stress fracture risk among girls participating in at least 1 h/d of high-impact activity, among whom 90.0% of the stress fractures occurred, and found that higher vitamin D intake predicted significantly lower risk of stress fracture (Ptrend = .04). CONCLUSIONS Vitamin D intake is associated with lower stress fracture risk among adolescent girls who engage in high levels of high-impact activity. Neither calcium intake nor dairy intake was prospectively associated with stress fracture risk.