Nitric oxide production mediates oligogalacturonide-triggered immunity and resistance to Botrytis cinerea in Arabidopsis thaliana

Plant Cell Environ. 2012 Aug;35(8):1483-99. doi: 10.1111/j.1365-3040.2012.02505.x. Epub 2012 Apr 4.


Nitric oxide (NO) regulates a wide range of plant processes from development to environmental adaptation. In this study, we investigated the production and/or function of NO in Arabidopsis thaliana leaf discs and plants elicited by oligogalacturonides (OGs) and challenged with Botrytis cinerea. We provided evidence that OGs triggered a fast and long lasting NO production which was Ca(2+) dependent and involved nitrate reductase (NR). Accordingly, OGs triggered an increase of both NR activity and transcript accumulation. NO production was also sensitive to the mammalian NO synthase inhibitor L-NAME. Intriguingly, we showed that L-NAME affected NO production by interfering with NR activity, thus questioning the mechanisms of how this compound impairs NO synthesis in plants. We further demonstrated that NO modulates RBOHD-mediated reactive oxygen species (ROS) production and participates in the regulation of OG-responsive genes such as anionic peroxidase (PER4) and a β-1,3-glucanase. Mutant plants impaired in PER4 and β-1,3-glucanase, as well as Col-0 plants treated with the NO scavenger cPTIO, were more susceptible to B. cinerea. Taken together, our investigation deciphers part of the mechanisms linking NO production, NO-induced effects and basal resistance to B. cinerea.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / immunology*
  • Botrytis / immunology
  • Botrytis / pathogenicity*
  • Calcium / metabolism
  • Hexuronic Acids / metabolism*
  • Ion Transport
  • Nitric Oxide / biosynthesis*
  • Reactive Oxygen Species / metabolism
  • Real-Time Polymerase Chain Reaction


  • Hexuronic Acids
  • Reactive Oxygen Species
  • Nitric Oxide
  • galacturonic acid
  • Calcium