DNA origami: synthesis and self-assembly

Curr Protoc Nucleic Acid Chem. 2012 Mar:Chapter 12:Unit 12.9.1-18. doi: 10.1002/0471142700.nc1209s48.


DNA origami is an emerging technology for designing defined two- and three-dimensional (2D and 3D) DNA nanostructures. Here, we report an introductory practical guide with step-by-step experimental details for the design and synthesis of origami structures, and their size expansion in 1D and 2D space by means of self-assembly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemical synthesis*
  • DNA / chemistry
  • DNA / isolation & purification
  • DNA / ultrastructure
  • Freeze Drying
  • Models, Molecular
  • Nanostructures / chemistry*
  • Nanotechnology / methods*
  • Nucleic Acid Conformation*
  • Particle Size
  • Temperature


  • DNA