Monodansylpentane as a blue-fluorescent lipid-droplet marker for multi-color live-cell imaging

PLoS One. 2012;7(3):e32693. doi: 10.1371/journal.pone.0032693. Epub 2012 Mar 1.


Lipid droplets (LDs) are dynamic cellular organelles responsible for the storage of neutral lipids, and are associated with a multitude of metabolic syndromes. Here we report monodansylpentane (MDH) as a high contrast blue-fluorescent marker for LDs. The unique spectral properties make MDH easily combinable with other green and red fluorescent reporters for multicolor fluorescence imaging. MDH staining does not apparently affect LD trafficking, and the dye is extraordinarily photo-stable. Taken together MDH represents a reliable tool to use for the investigation of dynamic LD regulation within living cells using fluorescence microscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Animals
  • Biological Transport
  • Biomarkers / metabolism
  • Contrast Media / pharmacology
  • Dansyl Compounds / pharmacology*
  • Fluorescent Dyes / pharmacology
  • Genetic Markers / genetics
  • HeLa Cells
  • Hep G2 Cells
  • Humans
  • Lipids / chemistry*
  • Mice
  • Microscopy, Fluorescence / instrumentation*
  • Microscopy, Fluorescence / methods*
  • Models, Biological
  • Models, Chemical
  • Spectrometry, Fluorescence / methods


  • Biomarkers
  • Contrast Media
  • Dansyl Compounds
  • Fluorescent Dyes
  • Genetic Markers
  • Lipids
  • monodansylpentane