Feeder cells support the culture of induced pluripotent stem cells even after chemical fixation

PLoS One. 2012;7(3):e32707. doi: 10.1371/journal.pone.0032707. Epub 2012 Mar 2.

Abstract

Chemically fixed mouse embryonic fibroblasts (MEFs), instead of live feeder cells, were applied to the maintenance of mouse induced pluripotent stem (miPS) cells. Formaldehyde and glutaraldehyde were used for chemical fixation. The chemically fixed MEF feeders maintained the pluripotency of miPS cells, as well as their undifferentiated state. Furthermore, the chemically fixed MEF feeders were reused several times without affecting their functions. These results indicate that chemical fixation can be applied to modify biological feeders chemically, without losing their original functions. Chemically fixed MEF feeders will be applicable to other stem cell cultures as a reusable extracellular matrix candidate that can be preserved on a long-term basis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / metabolism
  • Animals
  • Cell Culture Techniques
  • Cell Differentiation
  • Cell Separation
  • Coculture Techniques
  • Extracellular Matrix / metabolism
  • Feeder Cells / cytology*
  • Fibroblasts / cytology*
  • Flow Cytometry
  • Green Fluorescent Proteins / metabolism
  • Homeodomain Proteins / metabolism
  • Induced Pluripotent Stem Cells / cytology*
  • Mice
  • Microscopy, Fluorescence / methods
  • Nanog Homeobox Protein
  • Neurons / metabolism
  • Stem Cells / cytology

Substances

  • Homeodomain Proteins
  • Nanog Homeobox Protein
  • Nanog protein, mouse
  • Green Fluorescent Proteins
  • Alkaline Phosphatase