Nitric oxide-releasing nanoparticles accelerate wound healing in NOD-SCID mice

Nanomedicine. 2012 Nov;8(8):1364-71. doi: 10.1016/j.nano.2012.02.014. Epub 2012 Mar 7.

Abstract

Wound healing is a complex process, coordinated by various biological factors. In immunocompromised states wound healing can be interrupted as a result of decreased numbers of immune cells, impairing the production of effector molecules such as nitric oxide (NO). Therefore, topical NO-releasing platforms, such as diethylenetriamine (DETA NONOate), have been investigated to enhance wound healing. Recently, we demonstrated a nanoparticle platform that releases NO (NO-NPs) in a sustained manner, accelerating wound healing in both uninfected and infected murine wound models. Here, NO-NPs were investigated and compared to DETA NONOate in an immunocompromised wound model using non-obese, diabetic, severe combined immunodeficiency mice. NO-NP treatment accelerated wound closure as compared to controls and DETA NONOate treatment. In addition, histological assessment revealed that wounds treated with NO-NPs had less inflammation, more collagen deposition, and more blood vessel formation as compared to other groups, consistent with our previous data in immunocompetent animals. These data suggest that NO-NPs may serve as a novel wound-healing therapy in the setting of immunocompromised states associated with impaired wound healing.

From the clinical editor: Wound healing in an immunocompromised host is often incomplete and is a source of major concern in such conditions. This work demonstrates in a murine model that in these settings NO releasing nanoparticles significantly enhance wound healing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Collagen / metabolism
  • Female
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • Nanoparticles* / administration & dosage
  • Nanoparticles* / chemistry
  • Nitric Oxide* / administration & dosage
  • Nitric Oxide* / chemistry
  • Platelet Aggregation / drug effects
  • Skin / drug effects
  • Skin / pathology
  • Wound Healing / drug effects*
  • Wound Infection / drug therapy
  • Wound Infection / pathology

Substances

  • Nitric Oxide
  • Collagen