Fontimonas thermophila gen. nov., sp. nov., a moderately thermophilic bacterium isolated from a freshwater hot spring, and proposal of Solimonadaceae fam. nov. to replace Sinobacteraceae Zhou et al. 2008

Int J Syst Evol Microbiol. 2013 Jan;63(Pt 1):254-259. doi: 10.1099/ijs.0.037127-0. Epub 2012 Mar 9.

Abstract

A novel bacterial strain designated HA-01(T) was isolated from a freshwater terrestrial hot spring located at Hot Springs National Park, Arkansas, USA. Cells were Gram-negative-staining, rod-shaped, aerobic, chemo-organotrophic, oxidase- and catalase-positive, non-spore-forming and motile by means of a single polar flagellum. Growth occurred at 37-60 °C, with an optimum between 45 and 50 °C, and at pH 6.5-8.5, with an optimum between pH 6.5 and 7.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest relatives of strain HA-01(T) were Solimonas aquatica NAA-16(T) (93.8 %), Solimonas flava CW-KD 4(T) (94.1 %), Solimonas soli DCY12(T) (93.1 %), Solimonas variicoloris MN28(T) (94.0 %), Nevskia ramosa Soe1(T) (91.2 %) and Hydrocarboniphaga effusa AP103(T) (91.1 %). Major fatty acids consisted of C(16 : 0), iso-C(16 : 0), C(16 : 1)ω5c and summed feature 8 (C(18 : 1)ω9c, C(18 : 1)ω7c and C(18 : 1)ω6c). Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and the major isoprenoid quinone was Q-8. The DNA G+C content was 64.4 mol%. Based on phylogenetic, phenotypic and chemotaxonomic evidence, it is proposed that strain HA-01(T) represents a novel species in a new genus for which the name Fontimonas thermophila gen. nov., sp. nov. is proposed. The type strain of the type species is HA-01(T) (= DSM 23609(T) = CCUG 59713(T)). A new family, Solimonadaceae fam. nov., is also proposed to replace Sinobacteriaceae Zhou et al. 2008.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arkansas
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Fresh Water / microbiology*
  • Gammaproteobacteria / classification*
  • Gammaproteobacteria / genetics
  • Gammaproteobacteria / isolation & purification
  • Hot Springs / microbiology*
  • Molecular Sequence Data
  • Phylogeny*
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Quinones
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/JN415769