Peak and time-integrated shear rates independently predict flow-mediated dilation

Abstract

Background: To determine whether peak and time-integrated shear rates independently predict flow-mediated dilation (FMD).

Methods: Eleven physically active (25 ± 5 years old) male subjects were tested. FMD was defined as the shear rate-diameter relationship. Hierarchical linear modeling was used to estimate brachial artery diameter change with repeated measures of shear rate nested within each subject. Two models were tested: 1) FMD was induced using ischemia-induced hyperemia (2, 4, 6, and 10 minutes); and 2) FMD was induced following transient (ischemia) and steady-state (forearm heating and handgrip exercise) increases in shear rate. For both models we determined whether peak, in addition to time-integrated shear rates, explained a significant portion of variation for diameter change.

Results: Model 1: Time integrated shear rates explained most of the variation for diameter change. However, peak shear rate explained an additional significant portion of variation. Model 2: The transient condition resulted in significantly (p = 0.012) smaller diameter change per shear rate change than the steady-state condition. However, when specifying peak shear rate as a covariate, the difference between conditions became nonsignificant (p = 0.138).

Conclusions: Peak and time-integrated shear rates independently predict FMD. Future studies using the FMD test should consider both parameters.