12q13 fragility in a family with recurrent spontaneous abortions: expression of the fragile site under different culture conditions

Ann Genet. 1990;33(2):88-91.

Abstract

A fragile site at the 12q13 band was found in metaphases from lymphocyte cultures of three members of a family. A comparison of the frequency and expression of the fragile site was carried out on cells cultured in RPM-I 1640 with and without BrdU and in 199 media. The fragile site was not typically folate-sensitive, being expressed in standard medium as well as in cultures after exposure to BrdU.

Publication types

  • Case Reports

MeSH terms

  • Abortion, Habitual / etiology
  • Abortion, Habitual / genetics*
  • Adult
  • Bromodeoxyuridine / pharmacology
  • Cells, Cultured
  • Chromosome Aberrations / genetics*
  • Chromosome Deletion
  • Chromosome Disorders
  • Chromosome Fragile Sites
  • Chromosome Fragility*
  • Chromosomes, Human, Pair 12 / drug effects
  • Chromosomes, Human, Pair 12 / ultrastructure*
  • Culture Media / pharmacology
  • Culture Techniques / methods
  • Female
  • Humans
  • Lymphocytes / ultrastructure
  • Male
  • Pedigree
  • Pregnancy

Substances

  • Culture Media
  • Bromodeoxyuridine