Abstract
A fragile site at the 12q13 band was found in metaphases from lymphocyte cultures of three members of a family. A comparison of the frequency and expression of the fragile site was carried out on cells cultured in RPM-I 1640 with and without BrdU and in 199 media. The fragile site was not typically folate-sensitive, being expressed in standard medium as well as in cultures after exposure to BrdU.
MeSH terms
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Abortion, Habitual / etiology
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Abortion, Habitual / genetics*
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Adult
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Bromodeoxyuridine / pharmacology
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Cells, Cultured
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Chromosome Aberrations / genetics*
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Chromosome Deletion
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Chromosome Disorders
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Chromosome Fragile Sites
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Chromosome Fragility*
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Chromosomes, Human, Pair 12 / drug effects
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Chromosomes, Human, Pair 12 / ultrastructure*
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Culture Media / pharmacology
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Culture Techniques / methods
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Female
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Humans
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Lymphocytes / ultrastructure
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Male
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Pedigree
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Pregnancy
Substances
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Culture Media
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Bromodeoxyuridine