Selection and characterization of human respiratory syncytial virus escape mutants resistant to a polyclonal antiserum raised against the F protein

Arch Virol. 2012 Jun;157(6):1071-80. doi: 10.1007/s00705-012-1274-2. Epub 2012 Mar 14.

Abstract

A human respiratory syncytial virus (HRSV) neutralization escape mutant was obtained after 56 serial passages in the presence of a polyclonal antiserum raised against the F protein. Nucleotide sequence analysis of this escape mutant virus revealed two amino acid substitutions: Asn268Ile and Val533Met. When this virus was allowed to grow in the absence of the anti-F polyclonal serum, only the mutation Asn268Ile was stably maintained. Both the double and single escape mutant viruses lost reactivity with mAbs belonging to antigenic site II of the fusion protein of RSV. Mutation Asn268Ile has already been reported in RS viruses that are resistant to mAbs 47F and 11 and palivizumab (PZ). We have thus identified a novel mutation (Val533Met) in the transmembrane domain of the F protein that was selected under immune pressure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Antibodies, Viral / immunology*
  • Hep G2 Cells
  • Humans
  • Immune Evasion*
  • Mutation, Missense*
  • Neutralization Tests
  • Respiratory Syncytial Virus Infections / immunology
  • Respiratory Syncytial Virus Infections / virology*
  • Respiratory Syncytial Virus, Human / genetics*
  • Respiratory Syncytial Virus, Human / immunology
  • Respiratory Syncytial Virus, Human / isolation & purification
  • Viral Fusion Proteins / chemistry
  • Viral Fusion Proteins / genetics*
  • Viral Fusion Proteins / immunology*

Substances

  • Antibodies, Viral
  • F protein, human respiratory syncytial virus
  • Viral Fusion Proteins