Abstract
Staphylococcus aureus spreads on the surface of soft agar, a phenomenon we termed "colony spreading." Here, we found that S. aureus culture supernatant inhibited colony spreading. We purified δ-hemolysin (Hld, δ-toxin), a major protein secreted from S. aureus, as a compound that inhibits colony spreading. The culture supernatants of hld-disrupted mutants had 30-fold lower colony-spreading inhibitory activity than those of the parent strain. Furthermore, hld-disrupted mutants had higher colony-spreading ability than the parent strain. These results suggest that S. aureus negatively regulates colony spreading by secreting δ-hemolysin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Bacterial Proteins / pharmacology
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Culture Media, Conditioned / chemistry
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Culture Media, Conditioned / pharmacology
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Dose-Response Relationship, Drug
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Hemolysin Proteins / genetics
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Hemolysin Proteins / metabolism*
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Hemolysin Proteins / pharmacology
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Microbial Viability / drug effects
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Microbial Viability / genetics
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Molecular Sequence Data
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Mutation
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Staphylococcus aureus / genetics
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Staphylococcus aureus / growth & development*
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Staphylococcus aureus / metabolism*
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Time Factors
Substances
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Bacterial Proteins
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Culture Media, Conditioned
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Hemolysin Proteins
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delta hemolysin protein, Staphylococcus aureus