Failure of setting of mineral trioxide aggregate in the presence of fetal bovine serum and its prevention

J Endod. 2012 Apr;38(4):536-40. doi: 10.1016/j.joen.2011.12.005. Epub 2012 Jan 9.

Abstract

Introduction: The purpose of this study was to examine the effect of fetal bovine serum (FBS) on the setting of 2 white mineral trioxide aggregates (MTAs) and to determine if calcium chloride (CaCl(2)) alters the setting behavior of both MTAs under this condition.

Methods: Two types of MTA cements (ProRoot MTA [Dentsply Tulsa Dental, Tulsa, OK] and MTA-Angelus [Angelus Dental Solutions, Londrina, Brazil]) were mixed with either distilled water (DW) or 10% CaCl(2) solution. Mixed MTA was placed in a polyethylene mold, and each mold was then placed in saline or FBS-soaked Oasis. After storing for 4 days, the MTA samples were removed from the molds, and the microhardness was independently measured at 3 different levels: 0.5 mm, 2 mm, and 4 mm away from the bottom in contact with the immersion solution.

Results: None of the samples mixed with DW and exposed to FBS for 4 days had set at the 0.5-mm and 2-mm levels. CaCl(2) was effective in reducing (the 0.5-mm level) or eliminating (the 2- and 4-mm levels) the adverse effect of FBS. The groups with short-term exposure to FBS had the same surface microhardness as the groups stored in saline-soaked Oasis.

Conclusions: MTA did not set when the mixed MTA was exposed to FBS for 4 days. In these cases, 10% CaCl(2) helped the setting of MTA, and the effect was more pronounced in ProRoot MTA than in MTA-Angelus. In contrast, short-term exposure to FBS had minimal or no detrimental effects on the setting behaviors of both MTAs.

MeSH terms

  • Algorithms
  • Aluminum Compounds / chemistry*
  • Animals
  • Bismuth / chemistry
  • Calcium Chloride / chemistry
  • Calcium Compounds / chemistry*
  • Cattle
  • Dental Cements / chemistry
  • Drug Combinations
  • Hardness
  • Humidity
  • Materials Testing
  • Oxides / chemistry*
  • Pyrroles / chemistry
  • Root Canal Filling Materials / chemistry*
  • Serum / chemistry*
  • Silicates / chemistry*
  • Sodium Chloride / chemistry
  • Surface Properties
  • Temperature
  • Time Factors
  • Vinyl Compounds / chemistry
  • Water / chemistry

Substances

  • Aluminum Compounds
  • Calcium Compounds
  • Dental Cements
  • Drug Combinations
  • MTA-Angelus
  • Oxides
  • Pyrroles
  • Root Canal Filling Materials
  • Silicates
  • Vinyl Compounds
  • mineral trioxide aggregate
  • poly(divinyl-co-N-vinylpyrrolidinone)
  • Water
  • Sodium Chloride
  • Calcium Chloride
  • Bismuth