The mechanisms of injuries to the tibiofibular syndesmosis include isolated rupture and rupture in combination with ankle fractures. Current concepts of surgical treatment are fixation using bioabsorbable screws, syndesmotic stapling, syndesmotic hooks, and the widely used screw fixation. Postoperative care utilises passive motion of the ankle joint either with or without axial weight-bearing. The aim of our investigation was to quantify the motion of the mortise during axial load. Therefore, photoelastic tests, on the one hand, and biomechanical tests of cadaveric specimens, on the other, using axial loads of up to 2,000 N were used. Our photoelastic investigations showed force distribution through the screw into the cranial and caudal parts of the distal fibula. Biomechanical testing showed a progressive dehiscence in both ruptured and fixated specimens up to 2.89 (ruptured) and 2.42 mm (despite screw). Our findings strongly suggest a concept of partial weight-bearing at most to support regeneration of scar tissue and to prevent the appearance of instability in the ankle joint.