Graphene-based chemiluminescence resonance energy transfer for homogeneous immunoassay

ACS Nano. 2012 Apr 24;6(4):2978-83. doi: 10.1021/nn300684d. Epub 2012 Mar 21.

Abstract

We report on chemiluminescence resonance energy transfer (CRET) between graphene nanosheets and chemiluminescent donors. In contrast to fluorescence resonance energy transfer, CRET occurs via nonradiative dipole-dipole transfer of energy from a chemiluminescent donor to a suitable acceptor molecule without an external excitation source. We designed a graphene-based CRET platform for homogeneous immunoassay of C-reactive protein (CRP), a key marker for human inflammation and cardiovascular diseases, using a luminol/hydrogen peroxide chemiluminescence (CL) reaction catalyzed by horseradish peroxidase. According to our results, anti-CRP antibody conjugated to graphene nanosheets enabled the capture of CRP at the concentration above 1.6 ng mL(-1). In the CRET platform, graphene played a key role as an energy acceptor, which was more efficient than graphene oxide, while luminol served as a donor to graphene, triggering the CRET phenomenon between luminol and graphene. The graphene-based CRET platform was successfully applied to the detection of CRP in human serum samples in the range observed during acute inflammatory stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • C-Reactive Protein / analysis
  • Energy Transfer*
  • Graphite / chemistry*
  • Hepatocytes / metabolism
  • Humans
  • Immunoassay / methods*
  • Luminescent Measurements / methods*
  • Models, Molecular
  • Molecular Conformation

Substances

  • Graphite
  • C-Reactive Protein