Pomological features, nutritional quality, polyphenol content analysis, and antioxidant properties of domesticated and 3 wild ecotype forms of raspberries (Rubus idaeus L.)

J Food Sci. 2011 May;76(4):C585-93. doi: 10.1111/j.1750-3841.2011.02142.x.

Abstract

The raspberry (Rubus idaeus L.) is an economically important berry crop that contains many phenolic compounds with potential health benefits. In this study, important pomological features, including nutrient content and antioxidant properties, of a domesticated and 3 wild (Yayla, Yavuzlar, and Yedigöl) raspberry fruits were evaluated. Also, the amount of total phenolics and flavonoids in lyophilized aqueous extracts of domesticated and wild ecotypes of raspberry fruits were calculated as gallic acid equivalents (GAEs) and quercetin equivalents (QE). The highest phenolic compounds were found in wild Yayla ecotype (26.66 ± 3.26 GAE/mg extract). Whilst, the highest flavonoids were determined in wild Yedigöl ecotype (6.09 ± 1.21 QA/mg extract). The antioxidant activity of lyophilized aqueous extracts of domesticated and wild ecotypes of raspberry fruits were investigated as trolox equivalents using different in vitro assays including DPPH(•), ABTS(•+), DMPD(•+), and O(•-)(2) radical scavenging activities, H(2)O(2) scavenging activity, ferric (Fe(3+)) and cupric ions (Cu(2+)) reducing abilities, ferrous ions (Fe(2+)) chelating activity. In addition, quantitative amounts of caffeic acid, ferulic acid, syringic acid, ellagic acid, quercetin, α-tocopherol, pyrogallol, p-hydroxybenzoic acid, vanillin, p-coumaric acid, gallic acid, and ascorbic acid in lyophilized aqueous extracts of domesticated and wild ecotypes of raspberry fruits were detected by high-performance liquid chromatography and tandem mass spectrometry (LC-MS-MS). The results clearly show that p-coumaric acid is the main phenolic acid responsible for the antioxidant and radical scavenging activity of lyophilized aqueous extracts of domesticated and wild ecotypes of raspberry fruits.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / analysis*
  • Ascorbic Acid / analysis
  • Benzaldehydes / analysis
  • Benzothiazoles / analysis
  • Benzothiazoles / metabolism
  • Chromatography, High Pressure Liquid / methods
  • Coumaric Acids / analysis
  • Ecotype*
  • Ellagic Acid / analysis
  • Fruit / chemistry*
  • Gallic Acid / analogs & derivatives
  • Gallic Acid / analysis
  • Hydrogen Peroxide / analysis
  • Hydrogen Peroxide / metabolism
  • Hydroxybenzoates / analysis
  • Plant Extracts / analysis*
  • Plant Extracts / chemistry
  • Polyphenols / analysis*
  • Propionates
  • Pyrogallol / analysis
  • Rosaceae / chemistry*
  • Sulfonic Acids / analysis
  • Sulfonic Acids / metabolism
  • Tandem Mass Spectrometry / methods

Substances

  • Antioxidants
  • Benzaldehydes
  • Benzothiazoles
  • Coumaric Acids
  • Hydroxybenzoates
  • Plant Extracts
  • Polyphenols
  • Propionates
  • Sulfonic Acids
  • Pyrogallol
  • Ellagic Acid
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • Gallic Acid
  • ferulic acid
  • Hydrogen Peroxide
  • vanillin
  • syringic acid
  • phenolic acid
  • trans-3-(4'-hydroxyphenyl)-2-propenoic acid
  • Ascorbic Acid