Changes of hydrogen peroxide and radical-scavenging activity of raspberry during osmotic, convective, and freeze-drying

J Food Sci. 2011 May;76(4):C663-8. doi: 10.1111/j.1750-3841.2011.02144.x. Epub 2011 Apr 14.


This study was conducted to investigate the influence of different drying treatments on antioxidant (AO) activity and phenolic content of raspberry (Rubus idaeus), cultivar Willamette. Whole raspberry fruits were dried convectively (air-drying), osmotically, and freeze-dried. Acetone-water extracts of fresh and dried raspberries were assessed for total phenolic content by standard Folin-Ciocalteau method. Two AO assays were applied, a recently developed direct current (DC) polarographic assay based on decrease of anodic oxidation current of hydrogen peroxide and widely used radical scavenge against the 1,1-diphenyl-2-picrylhydrazyl (DPPH). Strong correlation has been obtained between both AO assays and total phenolic content. In addition, some individual phenolic compounds present in raspberry have been assessed using DPPH and DC polarographic assay. Comparison and evaluation of drying methods has been based on preservation of AO activity and total phenolic content. Obtained results confirmed superiority of freeze-drying; convective drying caused slight changes while osmotic dehydration showed a significant decrease of phenolic compounds and AO activity.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biphenyl Compounds / metabolism
  • Desiccation
  • Food Handling / methods*
  • Free Radical Scavengers / analysis
  • Free Radical Scavengers / chemistry*
  • Freeze Drying / methods*
  • Fruit / chemistry*
  • Hydrogen Peroxide / analysis
  • Hydrogen Peroxide / chemistry*
  • Molybdenum
  • Osmosis
  • Oxidation-Reduction
  • Phenols / analysis
  • Phenols / metabolism
  • Picrates / metabolism
  • Rosaceae / chemistry
  • Tungsten Compounds


  • Biphenyl Compounds
  • Folin's phenol reagent
  • Free Radical Scavengers
  • Phenols
  • Picrates
  • Tungsten Compounds
  • Molybdenum
  • Hydrogen Peroxide
  • 1,1-diphenyl-2-picrylhydrazyl