GC-MS based metabolomics for rapid simultaneous detection of Escherichia coli O157:H7, Salmonella Typhimurium, Salmonella Muenchen, and Salmonella Hartford in ground beef and chicken

J Food Sci. 2011 May;76(4):M238-46. doi: 10.1111/j.1750-3841.2011.02132.x. Epub 2011 Apr 13.


A metabolomic-based method for rapid detection of Escherichia coli O157:H7, Salmonella Hartford, Salmonella Typhimurium, and Salmonella Muenchen in nonselective media was developed. All pathogenic bacteria were grown in tryptic soy broth (TSB) at 37 °C followed by metabolite quantification at 2-h intervals for 24 h. Results were compared with the metabolite profiles similarly obtained with E. coli K12, Pseudomonas aeruginosa, Staphylococcus aureus, Saccharomyces cereviseae, and Aspergillus oryzae grown individually or as a cocktail under the same conditions. Principal component analysis (PCAS) discriminated pathogenic microorganisms grown in TSB. Metabolites responsible of PCAS classification were dextrose, cadaverine, the aminoacids L-histidine, glycine, and L-tyrosine, as well as the volatiles 1-octanol, 1-propanol, 1butanol, 2-ethyl-1-hexanol, and 2,5-dimethyl-pyrazine. Partial least square (PLS) models based on the overall metabolite profile of each bacteria were able to detect the presence of Escherichia coli O157:H7 and Salmonella spp. at levels of approximately 7 ± 2 CFU/25 g of ground beef and chicken within 18 h.

MeSH terms

  • Animals
  • Cattle
  • Chickens
  • Escherichia coli O157 / growth & development
  • Escherichia coli O157 / isolation & purification*
  • Food Contamination / analysis*
  • Food Microbiology / methods
  • Gas Chromatography-Mass Spectrometry / methods*
  • Meat / microbiology*
  • Metabolomics / methods*
  • Principal Component Analysis
  • Reproducibility of Results
  • Salmonella / classification
  • Salmonella / growth & development
  • Salmonella / isolation & purification*