[Obtaining of hairy-root, callus and suspension carrot culture (Daucus carota L.) able to accumulate human interferon alpha-2b]

Tsitol Genet. 2012 Jan-Feb;46(1):18-26.
[Article in Ukrainian]


Here we report the obtaining of suspension, callus and hairy root culture initiated from carrot plants of Nantskaya and Perfektzya variety with the highest level of recombinant human interferon alpha-2b accumulation exhibited the highest level of plant protein extract antiviral activity (up to 12.8 x 10(3) IU/mg TSP). The antiviral activity of callus extracts was significantly lower comparing to the activity of plant extracts from parent organisms. However, the antiviral activity level of suspension culture extracts (up to 4.42 x 10(3) IU/mg TSP) and Ri-root ones (up to 4.42 x 10(3) IU/mg TSP) appeared to be comparable to analogical data of antiviral activity of transgenic carrot leaf extracts, this way the described cultures could be possibly used for comparatively speedy obtaining of recombinant therapeutic protein for curing and preventing of virus diseases.

MeSH terms

  • Antiviral Agents / metabolism
  • Cells, Cultured
  • Daucus carota / genetics
  • Daucus carota / metabolism*
  • Genetic Variation
  • Genotype
  • Humans
  • Interferon alpha-2
  • Interferon-alpha / biosynthesis*
  • Interferon-alpha / genetics
  • Plant Extracts / metabolism
  • Plant Leaves / chemistry
  • Plant Roots / metabolism
  • Plants, Genetically Modified
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics
  • Virus Diseases / drug therapy


  • Antiviral Agents
  • Interferon alpha-2
  • Interferon-alpha
  • Plant Extracts
  • Recombinant Proteins