Comment on "Widespread RNA and DNA sequence differences in the human transcriptome"

Science. 2012 Mar 16;335(6074):1302; author reply 1302. doi: 10.1126/science.1210484.


Li et al. (Research Articles, 1 July 2011, p. 53; published online 19 May 2011) reported more than 10,000 mismatches between messenger RNA and DNA sequences from the same individuals, which they attributed to previously unrecognized mechanisms of gene regulation. We found that at least 88% of these sequence mismatches can likely be explained by technical artifacts such as errors in mapping sequencing reads to a reference genome, sequencing errors, and genetic variation.

Publication types

  • Research Support, N.I.H., Extramural
  • Comment

MeSH terms

  • DNA / genetics*
  • Genetic Variation*
  • Genome, Human*
  • Humans
  • RNA, Messenger / genetics*


  • RNA, Messenger
  • DNA