Cep70 promotes microtubule assembly in vitro by increasing microtubule elongation

Acta Biochim Biophys Sin (Shanghai). 2012 May;44(5):450-4. doi: 10.1093/abbs/gms017. Epub 2012 Mar 16.

Abstract

Microtubules are dynamic cytoskeletal polymers present in all eukaryotic cells. In animal cells, they are organized by the centrosome, the major microtubule-organizing center. Many centrosomal proteins act coordinately to modulate microtubule assembly and organization. Our previous work has shown that Cep70, a novel centrosomal protein regulates microtubule assembly and organization in mammalian cells. However, the molecular details remain to be investigated. In this study, we investigated the molecular mechanism of how Cep70 regulates microtubule assembly using purified proteins. Our data showed that Cep70 increased the microtubule length without affecting the microtubule number in the purified system. These results demonstrate that Cep70 could directly regulate microtubule assembly by promoting microtubule elongation instead of microtubule nucleation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle Proteins
  • Centrosome / metabolism
  • Centrosome / physiology*
  • Humans
  • Microtubule-Associated Proteins / physiology*
  • Microtubule-Organizing Center / metabolism
  • Microtubules / metabolism
  • Microtubules / physiology*

Substances

  • Cell Cycle Proteins
  • Cep70 protein, human
  • Microtubule-Associated Proteins