Effect of RECK gene polymorphisms on hepatocellular carcinoma susceptibility and clinicopathologic features

PLoS One. 2012;7(3):e33517. doi: 10.1371/journal.pone.0033517. Epub 2012 Mar 12.


Background: The reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) down-regulation has been confirmed in numerous human cancers and is clinically associated with metastasis. This study investigates the potential associations of RECK single-nucleotide polymorphisms (SNPs) with hepatocellular carcinoma (HCC) susceptibility and its clinicopathologic characteristics.

Methodology/principal findings: A total of 135 HCC cancer patients and 501 cancer-free controls were analyzed for four RECK SNPs (rs10814325, rs16932912, rs11788747, and rs10972727) using real-time PCR and PCR-RFLP genotyping analysis. After adjusting for other co-variants, the individuals carrying RECK promoter rs10814325 inheriting at least one C allele had a 1.85-fold [95% confidence interval (CI), 1.03-3.36] risk of developing HCC compared to TT wild type carriers. The HCC patients, who carried rs11788747 with at least one G allele, had a higher distant metastasis risk than wild type probands.

Conclusions: RECK gene polymorphisms might be a risk factor increasing HCC susceptibility and distant metastasis in Taiwan.

MeSH terms

  • Carcinoma, Hepatocellular / epidemiology*
  • Carcinoma, Hepatocellular / genetics*
  • Case-Control Studies
  • DNA Primers / genetics
  • GPI-Linked Proteins / genetics*
  • Genetic Association Studies
  • Genetic Predisposition to Disease / genetics*
  • Humans
  • Liver Neoplasms / epidemiology*
  • Liver Neoplasms / genetics*
  • Logistic Models
  • Neoplasm Metastasis / genetics*
  • Polymorphism, Restriction Fragment Length / genetics
  • Polymorphism, Single Nucleotide / genetics
  • Real-Time Polymerase Chain Reaction
  • Risk Factors
  • Statistics, Nonparametric
  • Taiwan / epidemiology


  • DNA Primers
  • GPI-Linked Proteins
  • RECK protein, human