The protective effects of ketamine and propofol in obstructive jaundice: an experimental study

Bratisl Lek Listy. 2012;113(3):139-44. doi: 10.4149/bll_2012_034.

Abstract

Objective: In this study, we investigated the protective effects of frequently used intravenous anesthetics (ketamine, propofol, thiopental, and fentanyl) in oxidative stress in a rat liver model of obstructive jaundice.

Materials and methods: Thirty-two Wistar albino rats were divided into four groups in a randomized fashion. All rats were subjected to laparotomy, common bile duct ligation and severance on day 0. Following 7 days, laparotomy was again performed using ketamine, propofol, pentobarbital, or fentanyl anesthesia. After 2 hours, the animals were sacrificed and tissue specimens were acquired for histopathological scoring and determination of malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) activities.

Results: All rats demonstrated enlargement in the bile duct, obstructive jaundice, and histopathologic ductal proliferation. MDA and SOD levels were significantly lower in the ketamine group compared with the thiopental and fentanyl groups. CAT was significantly increased in the ketamine group compared with the other groups. The best portal polymorphonuclear leukocyte and necrosis scores were in the ketamine group, but this difference was not statistically significant ( p=0.07).

Conclusion: Ketamine and propofol were observed to cause the least amount of oxidative stress in this rat model of induced oxidative stress generated by ligation of the common bile duct. This experiment is the first study on this subject in the literature (Tab. 3, Ref. 65).

MeSH terms

  • Anesthetics, Dissociative / pharmacology*
  • Anesthetics, Intravenous / pharmacology*
  • Animals
  • Catalase / metabolism
  • Jaundice, Obstructive / metabolism*
  • Jaundice, Obstructive / pathology
  • Ketamine / pharmacology*
  • Liver / metabolism
  • Liver / pathology
  • Male
  • Malondialdehyde / metabolism
  • Oxidative Stress / drug effects
  • Propofol / pharmacology*
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / metabolism

Substances

  • Anesthetics, Dissociative
  • Anesthetics, Intravenous
  • Malondialdehyde
  • Ketamine
  • Catalase
  • Superoxide Dismutase
  • Propofol