Electron microscopic immunogold cytochemistry reveals chromogranin A confined to secretory granules of porcine Merkel cells

Neurosci Lett. 1990 Aug 24;116(3):245-9. doi: 10.1016/0304-3940(90)90081-j.


By ultrastructural immunohistochemistry using the immunogold technique, immunoreactive (ir) Chromogranin A (CGA) was found to be confined to the secretory vesicles of porcine Merkel cells. CGA was present predominantly in the periphery of the electron-dense core and on the clear halo. These findings indicate that CGA is a regular constituent of Merkel cell secretory granules but probably not exclusively responsible for their electron opacity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromogranin A
  • Chromogranins / metabolism*
  • Cytoplasmic Granules / metabolism*
  • Gold
  • Immunohistochemistry
  • Microscopy, Electron
  • Neurosecretory Systems / cytology
  • Neurosecretory Systems / metabolism*
  • Neurosecretory Systems / ultrastructure
  • Skin / cytology
  • Skin / metabolism
  • Skin / ultrastructure
  • Swine


  • Chromogranin A
  • Chromogranins
  • Gold