Covariance spectroscopy with a non-uniform and consecutive acquisition scheme for signal enhancement of the NMR experiments

Yixuan Li; Qiang Wang; Zhengfeng Zhang; Jun Yang; Bingwen Hu; Qun Chen; Isao Noda; Feng Deng

doi:10.1016/j.jmr.2012.02.016

Covariance spectroscopy with a non-uniform and consecutive acquisition scheme for signal enhancement of the NMR experiments

J Magn Reson. 2012 Apr:217:106-11. doi: 10.1016/j.jmr.2012.02.016. Epub 2012 Mar 7.

Authors

Yixuan Li¹, Qiang Wang, Zhengfeng Zhang, Jun Yang, Bingwen Hu, Qun Chen, Isao Noda, Feng Deng

Affiliation

¹ Physics Department & Shanghai Key Laboratory of Magnetic Resonance, East China Normal University, Shanghai 200062, China.

PMID: 22436466
DOI: 10.1016/j.jmr.2012.02.016

Abstract

Two-dimensional covariance (COV2D) spectroscopy with non-uniform and consecutive acquisition (NUCA) scheme is introduced. This NUCA-COV2D method allows the number of t(1) points to be reduced by a factor of 1.5-3 without any broadening of the linewidth. Furthermore, the signal-to-noise ratio (S/N) can be increased up to 50%, which can further save experimental time by another factor of 2. This method has been demonstrated with model samples and the microcrystalline proteins. In all cases, the total experimental time can be reduced by a factor of 3-6, without any loss of resolution and S/N, with respect to what is necessary with the FT2D NMR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms*
Magnetic Resonance Spectroscopy / methods*
Proteins / chemistry*
Proteins / ultrastructure*
Sensitivity and Specificity

Substances

Proteins