Ki-67 immunostaining in uveal melanoma. The effect of pre-enucleation radiotherapy

Ophthalmology. 1990 Oct;97(10):1275-80. doi: 10.1016/s0161-6420(90)32420-x.


The reactivity of 33 choroidal and ciliary body melanomas with monoclonal antibody Ki-67, which recognizes a proliferation associated nuclear antigen, has been assessed and compared with clinicopathologic parameters. In 23 cases, 8 Gy irradiation was given 2 days before enucleation. Nonirradiated melanomas had a significantly higher proliferation rate as defined by staining with monoclonal antibody Ki-67 as compared with irradiated tumors (P = 0.007). Similarly, a strong relationship was found between pre-enucleation irradiation and low mitotic activity (P = 0.001). There was no significant correlation between the presence of Ki-67-positive nuclei and histologic classification, largest tumor diameter, localization of the tumor, age, sex, scleral invasion, pigmentation, and lymphocytic infiltration. The relevance of Ki-67 immunohistochemistry for the assessment of the life prognosis of patients with uveal melanoma has to be studied prospectively.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Monoclonal / immunology*
  • Antibodies, Neoplasm / immunology*
  • Antigens, Neoplasm / immunology
  • Cell Division / radiation effects
  • Choroid Neoplasms / immunology
  • Choroid Neoplasms / pathology*
  • Choroid Neoplasms / radiotherapy
  • Ciliary Body / immunology
  • Ciliary Body / pathology*
  • Eye Enucleation
  • Humans
  • Melanoma / immunology
  • Melanoma / pathology*
  • Melanoma / radiotherapy
  • Uveal Neoplasms / immunology
  • Uveal Neoplasms / pathology*
  • Uveal Neoplasms / radiotherapy


  • Antibodies, Monoclonal
  • Antibodies, Neoplasm
  • Antigens, Neoplasm