Abstract
We previously constructed a recombinant monoclonal antibody (rec-MAb 63P4) that detects immediate-early protein IE63 encoded by varicella-zoster virus (VZV) in the cytoplasm of productively infected cells. Here, we used ORF63 truncation mutants to map the rec-MAb 63P4 binding epitope to amino acids 141 to 150 of VZV IE63, a region not shared with other widely used anti-IE63 antibodies, and found that the recombinant antibody does not bind to the simian IE63 counterpart.
Publication types
-
Research Support, N.I.H., Extramural
MeSH terms
-
Amino Acid Sequence
-
Antibodies, Monoclonal / immunology*
-
Antibodies, Viral / immunology*
-
Epitope Mapping
-
Epitopes, B-Lymphocyte / immunology*
-
Herpesvirus 3, Human / genetics
-
Herpesvirus 3, Human / immunology*
-
Humans
-
Immediate-Early Proteins / genetics
-
Immediate-Early Proteins / immunology*
-
Molecular Sequence Data
-
Sequence Deletion
-
Sequence Homology, Amino Acid
-
Viral Envelope Proteins / genetics
-
Viral Envelope Proteins / immunology*
Substances
-
Antibodies, Monoclonal
-
Antibodies, Viral
-
Epitopes, B-Lymphocyte
-
Immediate-Early Proteins
-
Viral Envelope Proteins
-
immediate early protein 63, Human herpesvirus 3