Protective Effect of Grape Seed Extract Against Oxidative Stress-Induced Cell Death in a Staurosporine-Differentiated Retinal Ganglion Cell Line

Curr Eye Res. 2012 Apr;37(4):339-44. doi: 10.3109/02713683.2011.645106.

Abstract

Objective: Grape seed extract (GSE) is a potent antioxidant. We examined the effect of GSE on oxidative stress-induced cell death in a transformed retinal ganglion cell line, RGC-5.

Methods: Staurosporine-differentiated RGC-5 (ssdRGC-5) cells obtained by treating RGC-5 cells with 1 µM staurosporine were incubated with GSE for 2 h and then exposed to buthionine sulfoximine plus glutamate (B/G) for 24 h. Cell death was detected using the LIVE/DEAD viability assay and the type of cell death was evaluated using fluorescein isothiocyanate-conjugated Annexin-V/propidium iodide staining. To investigate the mechanism underlying cell death, we determined the caspase-3 activity and level of reactive oxygen species (ROS) formation.

Results: Treatment of ssdRGC-5 cells with B/G increased intracellular ROS and induced apoptosis (not necrosis) with increasing caspase-3 activity. GSE rescued the ssdRGC-5 cells from oxidative stress-induced cell death by inhibiting both intracellular ROS production and caspase-3 activation.

Conclusion: GSE had a neuroprotective effect against oxidative stress-induced apoptotic death in ssdRGC-5 cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Caspase 3 / drug effects
  • Caspase 3 / metabolism
  • Cell Death / drug effects*
  • Cell Line
  • Culture Media
  • Enzyme Inhibitors / pharmacology
  • Fluorometry
  • Grape Seed Extract / pharmacology*
  • Humans
  • Intracellular Fluid / metabolism
  • Oxidative Stress / drug effects*
  • Reactive Oxygen Species / metabolism
  • Retinal Ganglion Cells / drug effects
  • Retinal Ganglion Cells / pathology*
  • Staurosporine / pharmacology

Substances

  • Antioxidants
  • Culture Media
  • Enzyme Inhibitors
  • Grape Seed Extract
  • Reactive Oxygen Species
  • Caspase 3
  • Staurosporine