Derivation, expansion and differentiation of induced pluripotent stem cells in continuous suspension cultures

Nat Methods. 2012 Mar 25;9(5):509-16. doi: 10.1038/nmeth.1939.


We describe derivation of induced pluripotent stem cells (iPSCs) from terminally differentiated mouse cells in serum- and feeder-free stirred suspension cultures. Temporal analysis of global gene expression revealed high correlations between cells reprogrammed in suspension and cells reprogrammed in adhesion-dependent conditions. Suspension culture-reprogrammed iPSCs (SiPSCs) could be differentiated into all three germ layers in vitro and contributed to chimeric embryos in vivo. SiPSC generation allowed for efficient selection of reprogramming factor-expressing cells based on their differential survival and proliferation in suspension culture. Seamless integration of SiPSC reprogramming and directed differentiation enabled scalable production of beating cardiac cells in a continuous single cell- and small aggregate-based process. This method is an important step toward the development of robust PSC generation, expansion and differentiation technology.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques / methods*
  • Cell Differentiation / physiology
  • Cellular Reprogramming / physiology
  • Chimera / physiology
  • Fibroblasts / cytology*
  • Induced Pluripotent Stem Cells / cytology*
  • Mice
  • Myocytes, Cardiac / cytology*

Associated data

  • GEO/GSE35422