A highly sensitive two-photon fluorescent probe for mitochondrial zinc ions in living tissue

Chem Commun (Camb). 2012 May 14;48(38):4546-8. doi: 10.1039/c2cc31077e. Epub 2012 Mar 27.

Abstract

We report a highly sensitive two-photon probe (SZn2-Mito) which shows a 70-fold two-photon excited fluorescence enhancement in response to Zn(2+) and can selectively detect mitochondrial Zn(2+) in a rat hippocampal slice at a depth of 100-200 μm by using two-photon microscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Hippocampus / metabolism
  • Humans
  • Infrared Rays
  • Ions / chemistry
  • Microscopy, Fluorescence*
  • Mitochondria / metabolism*
  • Photons
  • Rats
  • Zinc / analysis*

Substances

  • Fluorescent Dyes
  • Ions
  • Zinc