A label-free fluorescence DNA probe based on ligation reaction with quadruplex formation for highly sensitive and selective detection of nicotinamide adenine dinucleotide

Chem Commun (Camb). 2012 May 11;48(37):4468-70. doi: 10.1039/c2cc30540b. Epub 2012 Mar 29.

Abstract

A simple label-free fluorescent sensing scheme for sensitive and selective detection of nicotinamide adenine dinucleotide (NAD(+)) has been developed based on DNA ligation reaction with ligand-responsive quadruplex formation. This approach can detect 0.5 nM NAD(+) with high selectivity against other NAD(+) analogs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Biosensing Techniques / methods*
  • DNA / chemistry*
  • DNA / genetics
  • Fluorescent Dyes / chemistry*
  • G-Quadruplexes*
  • NAD / analysis*
  • NAD / chemistry

Substances

  • Fluorescent Dyes
  • NAD
  • DNA