A pilot study of the immunological effects of high-dose vitamin D in healthy volunteers

Mult Scler. 2012 Dec;18(12):1797-800. doi: 10.1177/1352458512442992. Epub 2012 Mar 28.


Although vitamin D deficiency is considered an environmental factor in multiple sclerosis (MS), the immunological and clinical effects of vitamin D supplementation remain unclear. We performed a pilot study of the immunomodulatory effects of vitamin D in healthy individuals (n=4), who took 5000-10,000 IU/day of vitamin D over 15 weeks. After 15 weeks of vitamin D supplementation, serum 25(OH) vitamin D levels rose significantly from baseline, with a corresponding increase in IL-10 production by peripheral blood mononuclear cells and a reduced frequency of Th17 cells. These data provide a strong rationale for randomised trials to assess the clinical effects of vitamin D supplementation in MS.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Interleukin-10 / biosynthesis
  • Leukocytes, Mononuclear / drug effects*
  • Pilot Projects
  • Th17 Cells / drug effects
  • Vitamin D / administration & dosage*
  • Vitamin D / blood
  • Vitamins / administration & dosage*
  • Vitamins / blood


  • Vitamins
  • Interleukin-10
  • Vitamin D